Extracellular vesicles (EVs) are nanosized transporters containing a diverse cargo from lipids, proteins to nucleic acids and other biomolecules. EVs can be used as carriers for therapeutics but are also therapeutics themselves. Milk extracellular vesicles (mEVs) derived from bovine and human breast milk can endure the harsh digestive conditions in our stomach and survive the digestive journey to reach the colon exerting their beneficial effects. Among those effects are the strengthening and restoring of mucosal, epithelial, and immune barrier integrity. This prevents toxins from reaching the bloodstream and the liver, alleviating intestinal dysfunction, inflammatory bowel disease, and liver inflammations. mEVs also stimulate the growth of beneficial microorganisms such as bifidobacteria, helping to restore a healthy microbiome. mEVs can also be used as a delivery system for medications. For a sustainable and cost-effective approach mEVs can be extracted out of whey of milk production processes and could be an interesting way to upcycle this “waste product”.