Genetic code expansion allows the incorporation of non-canonical amino acids (ncAAs) into defined positions of proteins. This requires an orthogonal aminoacyl-tRNA synthetase and tRNA pair that does not cross-react with the host’s own translational machinery.

This project develops a new biomanufacturing strategy to make recovery of intracellular metabolites simpler and more economical. The cell membrane is a bottleneck for product recovery as the lipid bilayer is built to retain metabolites inside the cell.