Understanding molecular details of proteins is critical for sucessful drug discovery, protein engineering or formulation development. However, traditional techniques often struggle to capture the dynamic nature of proteins in solution. Let us help you overcome this challenge using HDX-MS!
Background
The concept of protein structure-function correlation has stimulated and guided applied research over the past decades. However, a detailed structural characterization of many systems is challenged by their intrinsic conformational dynamics that complicate analyses using standard techniques like x-ray crystallography, NMR or cryo-EM. HDX-MS (Hydrogen-Deuterium-eXchange – Mass Spectrometry) offers a complementary approach by directly addressing the conformational dynamics of proteins and providing straight-forward access to the characterization of multiple functionally relevant states.
Technology
Offer
We offer a flexible approach to a range of scientific questions including, but not limited to:
- identification of ligand (drug) binding sites
- analysis of allosteric changes induced by drugs/ligands
- effects of post-translational modifications (heterogeneity, dynamics)
- protein-protein interaction interfaces
- analysis of conformational dynamics / assigning unstructured regions • effects of solvent, pH, buffer composition on protein dynamics
- effects of solvent, pH, buffer composition on protein dynamics
- identification of allosteric effects in mutational analyses of proteins
- epitope mapping
Ready to elucidate the hidden potential of your protein? Contact us to discuss specifically tailored approaches to your scientific question - from project planning, to measurement, to analysis and final reports. We will show you how HDX-MS can be beneficial for your research and development analyses, but also for routine measurements aiding e.g. the assessment of batch-to-batch reproducibility or ensemble heterogeneity in protein formulations.
Experts:
Dr. Andreas Winkler, Philipp PelzmannDevelopment status:
Technology Readiness Level 2-7 (Problem set dependent from technology concept formulated to complete established analytical workflow)
