Evaluation of Aspergillus-specific aptamers
Starting date: August/September 2022; Duration: 6 months
This thesis project focuses on the evaluation and use of DNA aptamers (short single-stranded oligonucleotides) for the recognition and binding of cells of the filamentous fungus Aspergillus niger. Aptamers specific to A. niger have the potential to be employed in diagnostic applications against aspergillosis.
By using state of the art techniques and instruments (e.g., qPCR, flow cytometry and cell sorting), you will test previously developed DNA aptamers for their capability to bind specifically to A. niger cells. In parallel, you will establish a method to evaluate fluorescent Aspergillus-specific aptamers based on the use of a fluorescence activated cell sorter (FACS).