The plant Nicotiana benthamiana (a close relative to the tobacco plant) is an efficient eukaryotic production-platform for a variety of recombinant proteins. The system is especially favoured for proteins that are difficult to express in bacterial hosts, as they provide intrinsic eukaryotic features. The recently optimized transient expression approach offers significant advantages in production speed (product can be harvested 4-6 days post DNA construct delivery), flexibility and expression level.
acib has longstanding experience with N. benthamiana-based recombi‐ nant protein production yielding up to 50% of total soluble protein. The system works exceedingly well for antibodies (e.g. 50-250 mg purified IgG per kg of plant material) and has been applied in emergency use, e.g. during the Ebola and SARS-CoV-2 outbreaks. The approach is suitable in producing highly complex proteins, such as oligomeric IgM and IgAs. We have the know-how and potential partners for up-scaling of protein expression. In addition, acib can offer a versatile glycol-engineered N. benthamiana platform for tailor-made glycoprotein production.
Experts:Prof. Dr. Herta Steinkellner, Prof. Dr. Richard Strasser
Investments, Joint Research Projects, Contract Research
Development status:Technology Readiness Level 1-5
IPR:Will be generated for our industrial partner / investor
Nicotiana benthamiana, Plant based expression, Transient expression, Glyco-Engineering, Antibodies