Identifying bioactive compounds in complex biological samples such as plant or fungal extracts is a challenging task, traditionally tackled with large scale extraction followed by extensive fractionation and purification of active ingredients from active fractions by crystallization and x-ray crystallography. However, modern OMIC approaches allow identification of active ingredient candidates directly in complex samples from minute amount of extracts.
We employ hydrophobic interaction liquid chromatography high resolution ion mobility mass spectrometry to identify differences between biologically active and inactive extracts in an untargeted manner. Candidate compounds can be classified into pre-existing compound classes according to their hydrophilicity, sum formula, collisional cross section and fragmentation mass spectra.
Experts:Dr. Matthias Schittmayer-Schantl
Joint Research Project, Contract Research