Lipid composition of membranes and storage lipids across different kingdoms of life can be surprisingly diverse. Many of these uncommon lipids have highly interesting properties as they might form micelles of different stabilities or membranes with special permeability properties. While in depth analysis of mammalian bulk lipids is well established, analytical technologies targeting speciality lipids as found in fungi and archaea are poorly developed.
We employ liquid chromatography high resolution ion mobility mass spectrometry to identify and quantify uncommon lipids from various hosts as required for metabolic engineering and production optimization of these compounds. So far, we have developed analytical workflows for uncommon sterol lipids and for ether lipids but are also available to expand to new targets.