Bioengineering of biological production systems either by traditional strain selection and adaption or by directed evolution approaches typically leads to systems either limited by the availability of individual synthetic precursors or by a general metabolic burden overload. Detailed analysis of the metabolic network allows identification of bottlenecks and side reactions, thereby pushing the production rate and product purity even further.
We employ hydrophobic interaction liquid chromatography high resolution mass spectrometry to quantify constituents of the major catabolic pathways and the energy and redox metabolome (A(cM,M,D,T)P, NAD(P)/H, Glutathione) in the relevant production hosts (bacterial, fungal and mammalian).
Experts:Dr. Matthias Schittmayer-Schantl
Joint Research Project, Contract Research