Yeasts are one of the most important microorganism used in the food, beverage and pharmaceutical industry. Therefore, the potential of increasing the productivity of yeasts such as Saccharomyces cerevisiae, Saccharomyces pastorianus and Pichia pastoris is gigantic. Aging affects the productivity of yeast cells and while old cells still consume nutrients the output is greatly reduced. The size of single cells as well as the composition of the cell membranes is changing with age, leading to different transport and productivity properties. By separating old yeast cells from young cells it is possible to rejuvenate the culture and boost productivity.
acib offers selective separation of yeast mother cells from daughter cells. A stationary phase material can be used for this separation operation. Expanded-bed chromatography is used to retain mother cells due to a specific affinity linker for the budding scars occurring in mother cells. While the daughter cells can be used for more effective fermentation processes, the retained mother cells can be eluted and reused as well. A faster growth of the cells as well as a higher productivity for ethanol production has been showed for Saccharomyces pastorianus. Such a separation is also possible for industrial workhorse Pichia pastoris. This will allow for higher productivity and subsequently lesser energy for the heating/cooling of yeasts in the fermentation processes. This method is an important step for more economical and sustainable yeast applications.
Experts:Prof. Dr. Sebastian Schwaminger
Available for:Joint Research Project, Contract Research, Investments
Technology Readiness Level 5 (Saccharomoyces cerevisiae and Saccharomoyces pastorianus)
Technology Readiness level 3 (Pichia pastoris)
IPR:Will be generated for you as our industrial partner / investor
Yeast, Beer, Pichia pastoris, Separation, Expanded Bed, Saccharomyces pastorianus, Pharmaceuticals Downstream Processing